Domain shift is a significant problem in histopathology. There can be large differences in data characteristics of whole-slide images between medical centers and scanners, making generalization of deep learning to unseen data difficult. To gain a better understanding of the problem, we present a study on convolutional neural networks trained for tumor classification of H&E stained whole-slide images. We analyze how augmentation and normalization strategies affect performance and learned representations, and what features a trained model respond to. Most centrally, we present a novel measure for evaluating the distance between domains in the context of the learned representation of a particular model. This measure can reveal how sensitive a model is to domain variations, and can be used to detect new data that a model will have problems generalizing to. The results show how learning is heavily influenced by the preparation of training data, and that the latent representation used to do classification is sensitive to changes in data distribution, especially when training without augmentation or normalization.

该论文研究了卷积神经网络在组织病理学中用于肿瘤分类的应用，分析了数据增强和归一化对模型性能和表示学习的影响，提出了衡量模型领域差异距离的新方法，结果表明训练数据的准备对学习结果有很大影响。

深度学习在组织病理学中的领域转移研究