Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation of cadaver tissue, as well as the resource-intensive nature of chemical staining procedures covering large tissue areas, which demand substantial labor, cost, and time. These challenges can become more pronounced during global health crises when the availability of histopathology services is limited, resulting in further delays in tissue fixation and more severe staining artifacts. Here, we report the first demonstration of virtual staining of autopsy tissue and show that a trained neural network can rapidly transform autofluorescence images of label-free autopsy tissue sections into brightfield equivalent images that match hematoxylin and eosin (H&E) stained versions of the same samples, eliminating autolysis-induced severe staining artifacts inherent in traditional histochemical staining of autopsied tissue. Our virtual H&E model was trained using >0.7 TB of image data and a data-efficient collaboration scheme that integrates the virtual staining network with an image registration network. The trained model effectively accentuated nuclear, cytoplasmic and extracellular features in new autopsy tissue samples that experienced severe autolysis, such as COVID-19 samples never seen before, where the traditional histochemical staining failed to provide consistent staining quality. This virtual autopsy staining technique can also be extended to necrotic tissue, and can rapidly and cost-effectively generate artifact-free H&E stains despite severe autolysis and cell death, also reducing labor, cost and infrastructure requirements associated with the standard histochemical staining.

通过虚拟染色技术，我们报告了尸检组织的首次实证，并展示了经过训练的神经网络可以快速将无标记尸检组织切片的自动荧光图像转化为与相同样品的苏木精和伊红(HE)染色版本相匹配的亮场等效图像，消除了传统组织化学染色中固有的由于尸体组织延迟固定引起的严重染色伪影，使其可以在新的经历严重自溶的尸检组织样品中有效地强调细胞核、细胞质和细胞外特征，该技术还可应用于坏死组织，能够快速且具有低成本地生成无伪影的HE染色，同时减少了与标准组织化学染色相关的劳动、成本和基础设施需求。

无标记尸检组织的虚拟组织学染色