Lateral flow immunoassays (LFIA) are widely used worldwide for the detection of different analytes because they combine multiple advantages such as low production cost, simplicity, and portability, which allows biomarkers detection without requiring infrastructure or highly trained personnel. Here we propose to provide solutions to the manufacturing process of LFIA at laboratory-scale, particularly to the controlled and active dispensing of the reagents in the form the Test Lines (TL) and the Control Lines (CL). To accomplish this task, we adapted a 3D printer to also control Syringe Pumps (SP), since the proposed adaptation of a 3D printer is easy, free and many laboratories already have it in their infrastructure. In turn, the standard function of the 3D printer can be easily restored by disconnecting the SPs and reconnecting the extruder. Additionally, the unified control of the 3D printer enables dual, active, regulable and simultaneous dispensing, four features that are typically found only in certain high-cost commercial equipment. With the proposed setup, the challenge of dispensing simultaneously at least 2 lines (CL and TL) with SPs controlled by a 3D printer was addressed, including regulation in the width of dispensed lines within experimental limits. Also, the construction of a LFIA for the detection of leptospirosis is shown as a practical example of automatized reagent dispensing.

通过用3D打印机控制注射泵实现可控、活性的试剂注入，提出了一个用于在实验室规模下制造侧向流免疫分析 (LFIA) 的解决方案，以及实例化的研究表明可自动完成病原体检测中的试剂注入。

3D printer-controlled syringe pumps for dual, active, regulable and
  simultaneous dispensing of reagents. Manufacturing of immunochromatographic
  test strips

用于双重、活性、可调、同时释放试剂的3D打印机控制注射泵。免疫色谱试纸的制造