The field of histology relies heavily on antiquated tissue processing and
staining techniques that limit the efficiency of pathologic diagnoses of cancer
and other diseases. Current staining and advanced labeling methods are often
destructive and mutually incompatible, requiring new tissue sections for each
stain. This prolongs the diagnostic process and depletes valuable biopsy
samples. In this study, we present an alternative label-free histology platform
using the first transmission-mode Photon Absorption Remote Sensing microscope.
Optimized for automated whole slide scanning of unstained tissue samples, the
system provides slide images at magnifications up to 40x that are fully
compatible with existing digital pathology tools. The scans capture high
quality and high-resolution images with subcellular diagnostic detail. After
imaging, samples remain suitable for histochemical, immunohistochemical, and
other staining techniques. Scattering and absorption (radiative and
non-radiative) contrasts are shown in whole slide images of malignant human
breast and skin tissues samples. Clinically relevant features are highlighted,
and close correspondence and analogous contrast is demonstrated with one-to-one
gold standard H&E stained images. Our previously reported pix2pix virtual
staining model is applied to an entire whole slide image, showcasing the
potential of this approach in whole slide label-free H&E emulation. This work
is a critical advance for integrating label-free optical methods into standard
histopathology workflows, both enhancing diagnostic efficiency, and broadening
the number of stains that can be applied while preserving valuable tissue
samples.

利用第一代透射式光子吸收遥感显微镜的标签自由组织学平台，以及 pix2pix 实验，全面提高了组织学诊断的效率和疾病诊断的准确性，同时允许光学方法（标签自由的）与传统染色工艺进行对比，应用广泛。